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Morphohistological study of the different constituents of a banana (Musa 'AAA', cv. 'Grande Naine') embryogenic cell suspension

Georget F., Domergue R., Ferrière N., Côte F.X.. 2000. Plant Cell Reports, 19 : p. 748-754.

Five types of cellular aggregates have been characterised in embryogenic cell suspensions of banana (Musa 'AAA' 'Grande naine' cv.). Type I corresponded to isolated cells or to small cell aggregates. Type II were composed of embryogenic cells. Type III can be distinguished from type II due to the presence of peripheral proliferation zones with embryonic cells. Type IV were composed of protodermic masses histologically comparable to proembryos. Type V were nodules composed of a central zone of meristematic cells and of an external zone of starchy cells. Each culture flask of a cell line contained a majority of one of the above-mentioned aggregate types. Histological studies of somatic embryo developement on semi-solid regeneration medium showed that there were close similarities between the initial steps of ontogenesis of the embryos and the different cell aggregates in liquid multiplication medium. It appeared that aggregates II-IV of the suspension belong to the same development continuum which reproduces the initial phases of somatic embryo ontogenesis on semi-solid medium. Type V resulted from the development of type IV, for which ontogenesis is hindered by direct contact with 2,4-dichlorophenoxyacetic acid and the shaken liquid multiplication medium. Type I aggregates probably do not belong to the development continuum but rather correspond to the degeneration of the other types of aggregates in the suspension. The presence of intermediate types in the liquid medium reinforces the hypothesis of a relationship between the aggregates. The aggregates tended to develop through time from a majority of type II or III at the beginning of their culture to types IV-V for older suspensions. (Résumé d'auteur)

Mots-clés : embryogénèse somatique; milieu de culture; culture de cellule; technique de culture; analyse de tissus; anatomie végétale; musa (bananes); histologie

Thématique : Multiplication végétative des plantes

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