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Review on the molecular tools for the understanding of the epidemiology of animal trypanosomis in West Africa

Duvallet G., De La Rocque S., Reifenberg J.M., Solano P., Lefrançois T., Bengaly Z., Sidibé I., Desquesnes M., Cuisance D., Cuny G.. 1998. In : 2nd World Meeting on Salivarian Trypanosomes, March 16 1998. Montpellier : CIRAD-EMVT, 4 p.. Internet Conference on Salivarian Trypanosomes. 2, 1998-03.

The epidemiology of animal trypanosomosis around Bobo-Dioulasso (Burkina Faso, West Africa) benefited a lot in the last years from the progress of molecular tools. The two most used molecular techniques were the Polymerase Chain Reaction (PCR) for the diagnosis of the disease in cattle and the characterization of the trypanosomes in the host and the vector on one hand, and the microsatellite DNA polymorphism in tsetse flies to study the intraspecific genetic variability of the vector on the other hand. The results obtained in the Sideradougou area during a recent two year survey with these techniques, associated with many other georeferenced informations conceming vector and cattle distribution, natural environnient, landuse, ground occupation, livestock management, were combined in a Geographical Information System (GIS). This new approach of a complex pathogenic system led to a better evaluation of the risk of trypanosome transmission.

Mots-clés : trypanosoma; distribution géographique; bétail; Épidémiologie; contrôle de maladies; plante indicatrice; glossina; adn; afrique occidentale

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