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Survival and growth of eastern white pine shoot apical meristems in vitro

Goldfarb B., Howe G.M., Hackett W.P., Monteuuis O.. 1996. Plant Cell, Tissue and Organ Culture, 46 (3) : p. 171-178.

DOI: 10.1007/BF02307092

Shoot apical meristems of seedling and masure eastern white pine trees were excised and grown in vitro. Placing the meristems on filters instead of directly on agarose-solidified nutrient medium enhanced survival of both juvenile and masure meristems. Applying forcing treatments to masure branches improved survival and growth of dissected meristems compared with mcristems from non-forccd hranches in experiments conducted over two years. No consistent differences were observed among 2-, 4-, and 6-wcek forcing trcatments. Including 5.37 nM (0.001 mg 1-1 ) I -naphthaleneacetic acid in the culture medium did not affect meristcm survival or growth. Some meristems from seedlings grew rapidly, produced primary leaves. underwent internode elongation, and in three cases, produced adventitious roots. Meristems from masure trees did not grow as rapidly as seedling meristems. The leaves produced by masure meristems appeared to be scale leaves and a tcw of these had brachyblast primordia in the exils. The shoots derived from masure meristems did not produce adventitious roots.

Mots-clés : pinus strobus; vitroplant; méristème apical; culture de méristème; croissance; viabilité; maturation; micropropagation

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