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Cirad

Creation of triploid citrus hybrids by electrofusion of haploid and diploid protoplasts

Ollitrault P., Dambier D., Vanel F.. 1998. In : FAO; RIAC. Taller biotecnologia aplicada al diagnostico y manejo de enfermedades en citricos = [Atelier sur les biotechnologies appliquées au diagnostic et au contrôle des maladies des agrumes]. Havana : FAO, p. 135-136. Redbio'98 : Latin American Meeting on Plant Biotechnology. 3, 1998-06-01/1998-06-05, La Habana (Cuba).

Seedlessness is a major criteria for the Citrus fresh fruit market. So triploidy breeding appears very promising for the selection of new citrus cultivars. In addition of spontaneous triploid selection and sexual hybridization between diploid and allotetraploid hybrids, CIRAD has developped a new method for triploid synthesis by somatic hybridization between diploid and haploid lines. This method enables to add without recombination, an haploid genome to the whole diploid genome of high organoleptic quality cultivars

Mots-clés : citrus; triploïdie; hybridation somatique; fusion de protoplastes; variété apyrène; numération cellulaire

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