Bacillus thuringiensis toxin gene expression in plants
Giband M., Mazier M., Tourneur J., Couzi P., Chaufaux J., Pannetier C.. 1997. In : BIOTEC; NSTDA; Agricultural Science Society of Thailand; Thaï Society for Biotechnology; Genetics Society of Thailand. Proceedings of the third Asia-Pacific conference on agricultural biotechnology : issues and choices. Thaïlande : BIOTEC, p. 335-339. Asia-Pacific Conference on Agricultural Biotechnology. 3, 1996-11-10/1996-11-15, Hua Hin (Thaïlande).
Plants expressing genes encoding -endotoxins (cry genes) from the bacterium Bacillus thuringiensis (Bt) have triggered great interest for the control of agronomically important insect pests. A wide variety of plant species has been transformed with genes coding for various toxins aimed mainly at Lepidopteran and Coleopteran pests. The first transformation experiments conducted with bacterial genes (wild-type or native genes) have shown that the level of expression of these genes in plants is usually too low to confer good protection. To circumvent these problems, Bt toxin genes have been partially modified or totally re-synthesized, dramatically improving their level of expression in plants. Despite these improvements, some problems remain. In particular, the control of less susceptible insects, and that of the safe deployment of transgenic plants in relation to the emergence of toxin-resistant insects has yet to be addressed.
Mots-clés : plante transgénique; bacillus thuringiensis; toxine bactérienne; expression des gènes; transformation génétique; lutte anti-insecte
Communication de congrès
Agents Cirad, auteurs de cette publication :
- Giband Marc — Bios / UMR AGAP