The strain 273 B, the type strain of a H serotype of Bacillus thuringiensis not yet characterized B. thuringiensis subsp. cameroun, serotype H32, was isolated from soil samples collected in Cameroon. This strain produces cuboidal parasporal bodies composed of two major proteins of 53 kDa and 35 kDa. N-terminal sequences of the major proteins share no homology with published sequences. Only the 35 kDa protein is susceptible in digestion by trypsin. A complex array of 9 plasmids was revealed.