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Occurrence of an isolate of maize stripe virus on sorghum in India

Peterschmitt M., Ratna A.S., Sacks W.R., Reddy D.V.R., Mughogho L.K.. 1991. Annals of Applied Biology, 118 (1) : p. 57-70.

A disease characterised by chlorotic stripes and bands, named sorghum stripe disease (SStD), was observed on sorghum in India with an incidence of less than 0.5% to nearly 10%. The affected plants were dwarfed and had poor or no panicle formation. This disease could be transmitted by the delphacid planthopper Peregrinus maidis to sorghum but not to Brachiaria eruciformis; Cenchrus ciliaris; Chloris barbata; Dichantium annulatum; Dichantium aristatum; Digitaria ciliaris; Dinebra retroflexa; Echinocloa colona; Eleusine coracana; Pennisetum glaucum; Pennisetum violaceum; Setaria pallida Fusca; Triticum aestivum and Zea mays. Sorghum stripe disease was shown to be caused by a tenuivirus serologically related to maize stripe virus (MStV). Virus particles were filamentous, less than 10 nm in width. The purified virus preparation contained only one polypeptide of 34 500 D. Eight species of nucleic acids, four ssRNA of 1.21, 0.87, 0.73, 0.47 x 10 (6)D and four dsRNA of 2.43, 1.69, 1.40, 0.71 x 10 (6)D, were extracted from purified virus preparations. When the four dsRNA were denatured, they migrated along with the four ssRNA species indicating that dsRNA contained duplex RNA of same molecular weight as the four ssRNA. In enzyme-linked immunosorbent assay and in electro-blot immunoassay it was evident that MStV-Sorg was serologically more closely related to the MStV isolates from Florida, Reunion and Venezuela than to a RStV isolate from Japan. The virus was named MStV-Sorg to distinguish it from MStV which readily infects maize. This is the first report of occurrence of a tenuivirus in the Indian subcontinent. (Résumé d'auteur)

Mots-clés : identification; sorghum; tenuivirus; inde; striure

Thématique : Maladies des plantes

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