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Influence of grafting technique on meristem micrografting of Douglas-fir

Monteuuis O.. 1995. New Forests, 10 (3) : p. 267-273.

DOI: 10.1007/BF00027928

Three techniques for in vitro micrografting of shoot meristems from a 15-year-old clone of Douglas fir (Pseudotsuga menziesii) were compared. Although micrografting was feasible with all three procedures, the success rate was greatly influenced by the type of graft used. The best score (50% success) was obtained when using a miniaturized side-grafting technique. The micrografts, which could be easily acclimatized to in vivo conditions, exhibited however substantial variability with regard to scion development.

Mots-clés : méristème; greffage; pseudotsuga menziesii

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