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Influence of the 20-kDa protein from Bacillus thuringensis sp. israelensis on the rate of production of truncated crylC proteins

Rang C., Bes M., Lullien-Pellerin V., Wu D., Federici B.A., Frutos R.. 1996. FEMS Microbiology Letters, 141 : p. 261-264.

DOI: 10.1111/j.1574-6968.1996.tb08395.x

The potential role of a molecular chaperone on the rate of production of extensively altered "Bacillus thuringiensis Cry I C proteins was investigated. Analysis of the proteins produced by the recombinant B. thuringiensis strains showed that the truncated proteins were produced at a low rate. Expression of the 20-kDa protein gene from B. thuringiensis sp. israelensis# in tandem with the truncated-crylC genes led to the production of a greater amount of proteins. The formation of inclusion bodies, however, did not occur even when the 20-kDa protein gene was expressed.

Mots-clés : bacillus thuringiensis; protéine microbienne; propriété biologique; lutte anti-insecte

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