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Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting

Gamiette F., Bakry F., Ano G.. 1999. Genetic Resources and Crop Evolution, 46 (1) : p. 19-27.

Chromosomes counting and flow cytometry were used for assessing the ploidy level of various yam accessions of the INRA collection located in Guadeloupe. About 85 different clones were evaluated in the D. alata and D. cayenensis-rotundata and four other wild species related to D. cayenensis-rotundata. All the studied clones fitted in 4x, 6x and 8x ploidy levels. No diploid clone was found. Chromosomes counts and flow cytometry data led to the same results. The flow cytometry histograms for D. cayenensis-rotundata were not separated from those of its related wild species. Polyploidisation by fusion of 2n+n garnets was found to be unlikely for the two species D. cayenensis-rotundata and D. alata. Moreover, these results lead to the conclusion that the D. cayenensis-rotundata cultigens and the wild species analysed in this study may belong to the same gene pool. (Résumé d'auteur)

Thématique : Génétique et amélioration des plantes

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