Publications des agents du Cirad

Cirad

Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso

Solano P., Michel J.F., Lefrançois T., De La Rocque S., Sididé I., Zoungrana A.. 1999. Veterinary Parasitology, 86 : p. 95-103.

DOI: 10.1016/S0304-4017(99)00137-5

African animal trypanosomoses constitute the most important vector-borne cattle diseases in sub-Saharan Africa. Generally it is considered that there is a great lack of accurate tools for the diagnosis of the disease. During a trypanosomosis survey in the agro-pastoral zone of Sideradougou, Burkina Faso, 1036 cattle were examined for trypanosomes using microscopy. The PCR was applied on a subset of 260 buffy-coat samples using primers specific for Trypanosoma congolense savannah and riverine-forest groups, T vivax, and T brucei. Parasitological examination and the molecular technique were compared, showing a better efficiency of the latter. In the near future, the PCR is likely to become an efficient tool to estimate the prevalence of African trypanosomoses in affected areas.

Mots-clés : protozoa; bovin; pcr; trypanosoma; trypanosoma congolense; trypanosoma vivax; trypanosoma brucei; trypanosomose; diagnostic; vecteur de maladie; burkina faso

Article (a-revue à facteur d'impact)

Agents Cirad, auteurs de cette publication :