Publications des agents du Cirad


Results of a Musa mapping project

Lagoda P., Noyer J.L., Baurens F.C., Grivet L., Raboin L.M., Amson C., Aubert G., Costet L., Lanaud C., Dambier D., Ganry J., Tézenas Du Montcel H., Horry J.P., Bakry F., Ollitrault P., Carreel F., Auboiron E., Fouré E., Jenny C., Tomekpé K., Grapin A., Faure A.S., Reschoeur F., Gonzales De Léon D.. 1998. In : IAEA ; FAO. Use of novel DNA fingerprinting techniques for the detection and characterization of genetic variation in vegetatively propagated crops. Proceedings. Vienna : IAEA, p. 35-43. (IAEA, 1047). FAO/IAEA Final Research Co-ordination Meeting, 1997-02-24/1997-02-28, Mumbai (Inde).

A completed map, based on two selfed progenies from two banana cultivars (M53 and SFB5) is presented (roughly 1200 cM). More than three hundred markers are linked in 11 linkage groups representing the genorne (2n=22) of Musa acuminata. Roughly one third of the markers are codominant restriction fragment polymorphisms (RFLPs; one hundred) or microsatellites (thirty). Two thirds of the markers are dominant amplified fragment length polymorphisms (AFLPs; 10% could be considered to be co-dominant). The mean linkage distance is 3 cM, but marker density still should be increased on a couple of linkage groups. Particularities for a mapping job in banana are discussed. Due to Musa acuminata sub-species specific translocations, up to 36% of all the markers tested show important segregation distortions. The need for a cooperative mapping initiative based on a proposed "frame-map" harbouring evenly spaced co-dominant "anchor" markers is proposed. CIRAD has published 45 sequence tagged microsatellite sites (STMS) in the EMBL database which are accessible at : "http : //' using the keywords LAGODA and MICROSATELLITE (EMBL accessions X87258 to X87265, X90740 to X90750 and Z85950 to Z85977).

Mots-clés : musa acuminata; carte génétique; génome; marqueur génétique

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