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PCR analysis and spatial repartition of trypanosomes infecting tsetse flies in Sideradougou area of Burkina Faso

De La Rocque S., Lefrançois T., Reifenberg J.M., Solano P., Kaboré I., Bengaly Z., Augusseau X., Cuisance D.. 1998. Annals of the New York Academy of Sciences, 849 : p. 32-38. Biennial Conference of the Society for Tropical Veterinary Medecine. 4, 1997-05-05/1997-05-09, Montpellier (France).

DOI: 10.1111/j.1749-6632.1998.tb11030.x

A parasitological and entomological survey was conducted in the Sideradougou area (south of Bobo Dioulasso, Burkina Faso) in order to identify transmission factors of African trypanosomosis. A total of 3600 tsetse flies (Glossina tachinoides, Glossina palpalis gambiensis) were captured along 120 km of linear gallery forest and half of them were dissected. PCR analysis was undertaken on parasitologically positive flies (161 G. tachinoides, 92 G. palpalis gambiensis) to characterize the different trypanosomes. All the results were integrated in a GIS (Geographical Information System). Spatial repartition of the characterized trypanosomes enabled to recognize different areas with specific patterns of infection. Relations with environmental factors are discussed.

Mots-clés : trypanosoma; maladie infectieuse; glossina tachinoides; glossina palpalis; distribution géographique; pcr; burkina faso

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