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Kinetics of cassava starch hydrolysis with Termamyl enzyme

Paolucci-Jeanjean D., Belleville M.P., Zakhia N., Rios G.M.. 2000. Biotechnology and Bioengineering, 68 (1) : p. 71-77.

Kinetic properties of Termamyl® 120L were investigated with respect to starch hydrolysis in a batch reactor at substrate concentrations of 50-270 g . dm-³ and enzyme concentrations of 0.17-1 cm³ . dm-³ (i.e., cm³ of Novo Nordisk enzymatic solution per dm-³ of raw cassava starch suspension). A general kinetic expression giving product concentration as a function of time was first developed; an equation relating the reaction rate of each product to the sum of the concentrations of oligosaccharides with a higher degree of polymerisation was then derived. The empirical model satisfactorily fits experimental data for oligosaccharides with a degree of polymerization ranging from 1 to 7. (Résumé d'auteur)

Mots-clés : bioréacteur; enzyme; modèle; amidon; hydrolyse enzymatique; manioc; amidon de manioc; cinétique

Thématique : Traitement et conservation des produits alimentaires

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