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The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice

Barakat A., Gallois P., Raynal M., Mestre-Ortega D., Sallaud C., Guiderdoni E., Delseny M., Bernardi G.. 2000. FEBS Letters (471) : p. 161-164.

DOI: 10.1016/S0014-5793(00)01393-4

Abstract Almost all the nuclear genes of four Gramineae (maize, wheat, barley, rice) and pea are located in DNA fractions covering only a 1-2% GC range and representing between 10 and 25% of the different genomes. These DNA fractions comprise large gene-rich regions (collectively called the 'gene space') separated by vast gene-empty, repeated sequences. In contrast, in Arabidopsis thaliana, genes are distributed in DNA fractions covering an 8% GC range and representing 85% of the genome. Here, we investigated the integration of a transferred DNA (T-DNA) in the genomes of Arabidopsis and rice and found different patterns of integration, which are correlated with the different gene distributions. While T-DNA integrates essentially everywhere in the Arabidopsis genome, integration was detected only in the gene space, namely in the gene-rich, transcriptionally active, regions of the rice genome. The implications of these results for the integration of foreign DNA are discussed.

Mots-clés : arabidopsis; oryza sativa; plante transgénique; transfert de gène; localisation de gène

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