Publications des agents du Cirad


Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso

Solano P., Michel J.F., De La Rocque S., Sidibé I., Cuny G., Duvallet G., Cuisance D.. 1999. In : CSIRTC. 25th meeting of the international scientific council for trypanosomiasis research and control, Mombasa (Kenya), 27 september-01 october 1999. s.l. : s.n., 1 p.. Réunion du Conseil Scientifique International pour la Recherche et la Lutte contre les Trypanosomiases (CSIRLT). 25, 1999-09-27/1999-10-01, Mombasa (Kenya).

During a trypanosomosis survey in the agro-pastoral zone of Sideradougou, Burkina Faso, 1036 heads of cattle were examined for trypanosomes using microscopy. The PCR was applied on a subset of 260 buffycoat samples using primers specific for Trypanosoma congolense savannah and riverine-forest groupe, T. vivax, and T. brucei. Parasitological examination and the moloculer technique were compared, showing a better efficiency of the latter. In the near future, the PCR is likely to become an efficient tool to estimate the prevalence of African trypanosomoses in affected areas.
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