Publications des agents du Cirad


Detection of the African swine fever genomic DNA on dried pig blood filter paper

Guy-Gonzague M., Roger F., Rousset D., Randriamparany T., Crucière C.. 2003. Journal of Applied Research in Veterinary Medicine, 1 (2) : p. 143-147.

Polymerase chain reaction (PCR) is one of the best techniques to detect hemadsorbing, non-hemadsorbing, and/or noncytopathic African swine fever (ASF) strains. In some circumstances, the collection, storage, and transport of biologic samples to an appropriate laboratory are not easy. For this purpose, the blood sampling on filter paper, a method widely used for the research of genomes by PCR, might be useful. This study has shown that ASFV DNA remains detectable by PCR after dry storage of blood on filter paper for at least 3 months at 30°C. Thus, the PCR was applied to filter paper blood samples from 62 pigs collected in Malagasy areas infected by ASFV The ASFV DNA was amplified for 34 of 62 dried blood samples. The results were similar to the previous ones obtained with fresh samples collected at the beginning of the epizootic.

Mots-clés : adn; pcr; virose; peste porcine africaine; épidémie

Article (b-revue à comité de lecture)

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