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Polyphasic characterization of xanthomonads isolated from onion, garlic and Welsh onion (Allium spp.) and their relatedness to different Xanthomonas species

Roumagnac P., Gagnevin L., Gardan L., Sutra L., Manceau C., Dickstein E.R., Jones J.B., Rott P., Pruvost O.. 2004. International Journal of Systematic and Evolutionary Microbiology, 54 : p. 15-24.

Bacterial blight is an emerging disease that affects primarily onion, but also garlic and Welsh onion. The present study was undertaken to characterize the causative xanthomonad(s) by a polyphasic approach using a worldwide collection of 33 bacterial strains. Analysis of 16S rRNA gene sequence similarities indicated that the causal agent belongs to the campestris core in the genus Xanthomonas, which is in agreement with results of phenotypic characterization (analyses of carbon source utilization and fatty acid methyl esters). However, DNA-DNA hybridization, thermal stability of DNA reassociation and fluorescent amplified fragment length polymorphism analysis allowed the causal agent to be identified as a pathovar of Xanthomonas axonopodis. (Résumé d'auteur)

Mots-clés : allium; xanthomonas; bactériose; identification; marqueur génétique; adn; arn ribosomal; xanthomonas axonopodis

Thématique : Maladies des plantes

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