Fourteen microsatellite loci of Phoenix dactylifera were targeted to examine the genetic diversity in Tunisian date-palms germplasm. They showed a high level of polymorphism in 49 accessions from three main oases with little geographic structure within Tunisia. The microsatellite data agrees with previous analyses of Tunisian germplasm using other molecular markers. 100% of local date-palms accessions were successfully fingerprinted and easily distinguished by the help of only three loci. The possibility of using microsatellites for large scale molecular labelling of offshoots and in vitro plantlets and their implication in the plant material certification is discussed.