Publications des agents du Cirad


Rapid determination of polyphenols and vitamin C in plant-derived products

Georgé S., Brat P., Alter P., Amiot M.J.. 2005. Journal of Agricultural and Food Chemistry, 53 (5) : p. 1370-1373.

DOI: 10.1021/jf048396b

Polyphenols, widely spread in our diet by the consumption of plant food products, are commonly determined using Folin-Ciocalteu reagent that interacts with other different reducing nonphenolic substances and leads to an overestimation of polyphenol content. In this paper we report an optimized Folin-Ciocalteu method to specifically determine the contents of total polyphenols and vitamin C. After the optimal conditions for the colorimetric assay were set, solid-phase extraction (Oasis HLB (hydrophilic-lipophilic balance)) was carried out to eliminate the water-soluble reducing interferences including vitamin C. Colorimetric correction was thus performed by subtracting interfering substances contained in the water washing extract from the raw extract. Moreover, vitamin C present in the water washing extract can be destroyed by heating and thus colorimetrically deduced. This procedure was set up with synthetic solutions and validated on different extracts from fruit products.

Mots-clés : fruits; pulpe de fruits; polyphénol; acide ascorbique; colorimétrie; purification; teneur en phénols; teneur en vitamines; méthode

Documents associés

Article (a-revue à facteur d'impact)

Agents Cirad, auteurs de cette publication :