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Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa

Alberto F., Correia L., Billot C., Duarte C.M., Serrao E.. 2003. Molecular Ecology Notes, 3 (3) : p. 397-399.

DOI: 10.1046/j.1471-8286.2003.00463.x

In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.

Mots-clés : microsatellite; marqueur génétique; identification; algue marine; cymodocea nodosa

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