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Induced spermiation and milt management in Pangasius bocourti (Sauvage, 1880)

Cacot P., Eeckhoutte P., Do Than Muon, Nguyen Viet Trieu, Legendre M., Mariojouls C., Lazard J.. 2003. Aquaculture, 215 (1-4) : p. 67-77.

DOI: 10.1016/S0044-8486(02)00032-7

Males of Pangasius bocourti (3.3-6.9 kg) raised in floating cages on the Mekong River were used to study the production of milt and its use for fertilization. Although spontaneous spermiation occurred in mature males, the average volume of collected milt was low (26.5 microl kg-1). A single human chorionic gonadotropin (hCG) injection (2000 IU kg-1) induced a 13-fold increase in sperm volume, 12 h after injection (344.5 microl kg-1). The associated sperm concentration and production were 26.9×109 spz ml-1 and 8.4×109 spz kg-1, respectively. Injection of GnRHa (30 microg kg-1) with domperidone (3 mg kg-1) induced a lower increase of sperm volume and sperm production. The motility of spermatozoa (spz) in well water lasted only 57 s, but was doubled in low saline solution (NaCl 34 mM). Crude milt could not be stored but five-fold dilution in immobilization solution (NaCl 207 mM) allowed fertilization to be postponed for 36 h. Fertilization was triggered by well water with milt diluted 1000 times. (Résumé d'auteur)

Mots-clés : pangasius; poisson chat (d'eau douce); reproduction; insémination artificielle

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