In the current context of overproduction and low prices, coffee producers give a particular attention to coffee quality, impact on human health being one of its components. In this context, diterpens content constitutes a relevant target trait (Cavin et al., 1998). Coffee diterpens (Cafestol, Kahweol and 16-O-Methylcafestol) are specific of the coffee genus(Finnegan et Djerassi, 1960; Speer and Mischnick, 1989). Little information is available on coffee diterpens metabolism. Today only the enzymes implied in the way of biosynthesis of Ent-Kaurene are known. The ultimate stages between Ent-Kaurene and Cafestol, Kahweol, 16-O-MC are not identified yet.