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Androgenic potential in coconut (Cocos nucifera L.)

Perera P.I.P., Hocher V., Verdeil J.L., Bandupriya D., Yakandawala D.M.D., Weerakoon L.K.. 2008. Plant Cell, Tissue and Organ Culture, 92 (3) : p. 293-302.

Conditions for induction of androgenesis in coconut cv. Sri Lanka Tall were studied. Anthers collected from inflorescences at four maturity stages were given heat (38_C) or cold (4_C) pretreatments for 1, 3, 6 and 14 days, either prior to or post inoculation. Three different basal media and different anther densities were also tested. Androgenesis was observed only in anthers collected from inflorescences 3 weeks before splitting (WBS) and after a heat pretreatment at 38_C for 6 days. Modified Eeuwens Y3 liquid medium supplemented with 100 lM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.1% activated charcoal and 9% sucrose was effective in inducing an androgenic response. The lowest anther density tested, 10 per petri plate, was found to be the optimal density. When androgenic calli or embryos were subcultured to Y3 medium containing 66 lM 2,4-D, followed by transfer to Y3 medium without plant growth regulators and finally to Y3 medium containing 5 lM 6-benzyladenine (BA) and 0.35 lM gibberellic acid (GA3), plantlets regenerated at a frequency of 7%. Histological study indicated that the calli and embryos originated from the inner tissues of the anthers. Ploidy analysis of calli and embryos showed that they were haploid. This is the first report of successful androgenesis yielding haploid plants from coconut anthers. (Résumé d'auteur)

Mots-clés : haploïdie; tissu végétal; développement biologique; numération cellulaire; embryon somatique; cal; androgénèse; culture d'anthère; cocos nucifera; plante haploïde

Thématique : Multiplication végétative des plantes; Physiologie végétale : croissance et développement

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