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Establishment of diversity arrays technology for whole-genome profiling of banana

Huttner E., Risterucci A.M., Hippolyte I., Caig V., Carlig J., Evers M., Uszynski G., Wenzl P., Glaszmann J.C., Kilian A.. 2007. In : Abstracts of Plant and Animal Genomes XVth Conference, San Diego, CA (USA), January 13-17, 2007. s.l. : s.n.. Plant and Animal Genomes Conference. 15, 2007-01-13/2007-01-17, San Diego (Etats-Unis).

The genetic improvement of ¿orphan¿ crops is going to benefit from modern breeding methods underpinned by molecular marker technologies. Establishing reliable associations between complex traits and genomic regions requires both accurate and comprehensive phenotypic characterization of the germplasm and cost-effective, high-density genotyping methods. Whole-genome, molecular profiles also enable breeders and scientists to better manage and utilize the available genetic diversity. Since many marker systems such as SSR and SNP depend on sequence information, marker discovery is still expensive and limits the development of whole-genome profiling tools for ¿orphan¿ crops. Diversity Arrays Technology (DArT) circumvents the need for sequence information and accelerates marker discovery and typing, both at a very low cost. We have established DArT for the Musa genus. Two arrays of 6,000 clones each are now available. On each array, 700 to 800 polymorphic markers were discovered in a broad survey of Musa genotypes. The majority of the markers separate the A and the B genomes, but subsets of markers resolve the diversity within each genome. A few markers also identify genetic heterogeneity within clones of cultivated banana. The low cost of DArT assays opens new opportunities for germplasm characterization and trait mapping in banana and plantain. In addition, the phenotypic effects of DNA methylation polymorphisms can now be identified and studied. The density of DArT molecular profiles will also benefit applications in Musa genomics. (Texte intégral)
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