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Characterization of Hawaiian isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid

Vaughan S.P., Grisoni M., Kumagai M.H., Kuehnle A.R.. 2008. Archives of Virology, 153 (6) : p. 1185-1189.

DOI: 10.1007/s00705-008-0102-1

We report here the isolation and characterization of three distinct isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid in Hawaii. Isolates 1 and 2 were phylogenetically distinct from previously reported CymMV isolates. However, isolate 3 was highly similar to previously reported CymMV sequences and could be localised to CymMV subgroup A. Isolate 2 localised to CymMV subgroup B. Thus, we report here the first full-length CymMV subgroup B isolate. Isolate 1 represents a recombination event between isolates 2 and 3. Infectivity assays revealed that all three isolates are functional and individually infectious in both Dendrobium and indicator species.

Mots-clés : virus des végétaux; potexvirus; dendrobium; identification; diagnostic; phylogénie; infection expérimentale; hawaï; cymbidium mosaic virus

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