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Decomposition of myceliar matrix and extraction of chitosan from Gongronella butleri USDB 0201 and Absidia coeruleareak ATCC 14076

Nwe N., Stevens W., Montet D., Tokura S., Tamura H.. 2008. International Journal of Biological Macromolecules, 43°1 : p. 2-7. International Conference on Chitin and Chitosan. 10, 2006-09-06/2006-09-09, Lyon (France).

Free chitosan, 2 g/100 g mycelia from Gongronella butleri and 6.5 g/100g mycelia from Absidia coerulea were isolated by 1M NaOH at 45 ?C for 13 h and 0.35M acetic acid at 95 ?C for 5 h. Both myceliar matrixes did not break down under these conditions. However, myceliar matrix could be decomposed by treatment with 11M NaOH at 45 ?C for 13 h and 0.35M acetic acid at 95 ?C for 5 h and then extracted the total chitosan, 8-9 g/100 g mycelia from both fungi. According to these results, G. butleri has higher amount of complexed chitosan and A. coerulea has higher amount in free chitosan. (Résumé d'auteur)

Mots-clés : mucorales; dégradation; extraction; hydroxyde de sodium; acide acétique; absidia; chitosane; gongronella butleri

Thématique : Traitement des produits agricoles non alimentaires

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