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Acetone powder from dormant seeds of Ricinus communis L : Lipase activity and presence of toxic and allergenic compounds

Cavalcanti E.D.C., Maciel F.M., Villeneuve P., Lago R.C.A., Machado O.L.T., Freire D.M.G.. 2007. Applied Biochemistry and Biotechnology, 137-140 (1-12) : p. 57-65. Symposium on Biotechnology for Fuels and Chemicals. 28, 2006-04-30/2006-05-03, Nashville (Etats-Unis).

DOI: 10.1007/s12010-007-9039-1

The influence of several factors on the hydrolytic activity of lipase, present in the acetone powder from dormant castor seeds (Ricinus communis) was evaluated. The enzyme showed a marked specificity for short-chain substrates. The best reaction conditions were an acid medium, Triton X-100 as the emulsifying agent and a temperature of 30°C. The lipase activity of the acetone powder of different castor oil genotypes showed great variability and storage stability of up to 90%. The toxicology analysis of the acetone powder from genotype Nordestina BRS 149 showed a higher ricin (toxic component) content, a lower 2S albumin (allergenic compound) content, and similar allergenic potential compared with untreated seeds.

Mots-clés : ricinus communis; estérase; activité enzymatique; graine; acétone; poudre; allergène; lipase

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