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Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera)

N'Nan O., Hocher V., Verdeil J.L., Konan Konan J.L., Ballo Koffi C., Mondeil F., Malaurie B.. 2008. CryoLetters, 29 (4) : p. 339-350.

This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.

Mots-clés : cocos nucifera; conservation biologique; congélation; encapsulation; déshydratation; plumule; tissu végétal; saccharose; régénération in vitro; pousse; cryoconservation; côte d'ivoire

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