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High-affinity copper transport and Snq2 export permease of Saccharomyces cerevisiae modulate cytotoxicity of PR-10 from Theobroma cacao

Pungartnik C., Da Silva A.C., Alves de Melo S., Peres Gramacho K., De Mattos Cascardo J.C., Brendel M., Micheli F., Da Silva Gesteira A.. 2009. Molecular Plant-Microbe Interactions, 22 (1) : p. 39-51.

A pathogenesis-related (PR) protein from Theobroma cacao (TcPR-10) was identified from a cacao-Moniliophthora perniciosa interaction cDNA library. Nucleotide and amino acid sequences showed homology with other PR-10 proteins having P loop motif and Betv1 domain. Recombinant TcPR-10 showed in vitro and in vivo ribonuclease activity, and antifungal activity against the basidiomycete cacao pathogen M. perniciosa and the yeast Saccharomyces cerevisiae. Fluorescein isothiocyanate-labeled TcPR-10 was internalized by M. perniciosa hyphae and S. cerevisiae cells and inhibited growth of both fungi. Energy and temperature-dependent internalization of the TcPR-10 suggested an active importation into the fungal cells. Chronical exposure to TcPR-10 of 29 yeast mutants with single gene defects in DNA repair, general membrane transport, metal transport, and antioxidant defenses was tested. Two yeast mutants were hyperresistant compared with their respective isogenic wild type: ctr3[delta] mutant, lacking the high-affinity plasma membrane copper transporter and mac1[delta], the copper-sensing transcription factor involved in regulation of high-affinity copper transport. Acute exposure of exponentially growing yeast cells revealed that TcPR-10 resistance is also enhanced in the Snq2 export permease-lacking mutant which has reduced intracellular presence of TcPR-10. (Résumé d'auteur)

Mots-clés : gène; toxicité; cuivre; génie génétique; résistance aux organismes nuisibles; expression des gènes; clonage moléculaire; protéine recombinante; moniliophthora; saccharomyces cerevisiae; theobroma cacao; moniliophthora perniciosa

Thématique : Génétique et amélioration des plantes; Maladies des plantes; Contamination et toxicologie alimentaires

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