Tlaxco is a small dairy area ubicated in the central part of Mexico. The different actors implicated in cheese value chain are concentrated in this zone: dairy farmers, milk collectors, cheese makers and sellers. The Local Agrifood System methodology has been applied to bring us a multidisciplinary point of view. Milk availability made possible cheese-making activity, and Tlaxco cheese enjoy a regional reputation. Actors are characterized by small scale activities and a low technological and economical level. Nevertheless, cheese-making activities are still in development. But relationships are limited: their main characteristics are mistrust, oportunism and individualism. Instead of promoting collectively product quality, competition is almost only based on price. The lack of colective activation in the area weakens actors and stops an optimal use of resources.