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Gene expression analysis for identifying candidate genes for controlling oxidative stress in rubber tree

Leclercq J., Gebelin V., Chabaud M., Rio M., Chrestin H., Montoro P.. 2009. In : 3rd IRRDB Workshop on the Hevea Genome and Transcriptome, 3-5 June, 2009, Montpellier, France. s.l. : s.n., p. 21-23. IRRDB Workshop on the Hevea Genome and Transcriptome. 3, 2009-06-03/2009-06-05, Montpellier (France).

Hevea brasiliensis is the main source of natural rubber which is biosynthesized in latex cells. The high metabolic productivity required for latex regeneration after each tapping can be enhanced by ethylene application, which optimizes the production potential in rubber tree. However, excessive metabolism activation can lead to Tapping Panel Dryness (TPD). Expression analyses of several genes involved in the reactive oxygen species (ROS) scavenging systems have been studied in healthy trees, TPD trees and also in young budded plants. Here, we presented the expression patterns obtained by semi-quantitative and real time RT-PCR of genes involved in ascorbate-glutathione cycle. Three genotypes (PB260, PB217 and RRIM600) with contrasting metabolism have been selected at the immature stage for this study. Their expression patterns have been monitored along the day, and in response to both ethylene stimulation and wounding treatments.

Mots-clés : hevea brasiliensis; guyane française; france; indonésie

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