Rubber breeding has long been based on conventional breeding and quantitative genetics based on phenotypic observations. Since recently, molecular genetic markers have brought new possibilities for characterizing genotypes, with view to identify cultivars, or their parents, to analyse genetic diversity, to establish relationships between agricultural traits and genetic factors (QTLs), and to identify genes of interest. In order to analyse the genetic bases of latex production, the cross RRIM600 x PB217 was created in Thailand for genotyping the progenies and building a genetic linkage map. The two parents were chosen for their contrasted physiological behaviour, as shown by the metabolic typology of cultivated rubber clones. Based on the double pseudo testcross strategy, two parental maps and a consensus map were built, with 334 progenies, 247 microsatellite markers, and 198 AFLP markers. The 18 linkage groups were assigned to the corresponding groups, equivalent to the 18 chromosomes of the Hevea haploid genome, of a Cirad Hevea genetic map used as reference. This new consensus map will be used together with field data for QTLs detection on heritable traits. Efforts will be priorily focussed on studying the physiological parameters of the latex diagnostic, in association with latex production.