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Immunodetection of legume proteins resistant to digestion in weaned piglets

Salgado P., Freire J.P.B., Lallès J.P.. 2004. In : Eds by M. Muzquiz, G.D. Hill, C. B. Recent advances of research in antinutritional factors in legume seeds and oilseeds. Proceedings of the 4th International Workshop on Antinutritional Factors in Legume Seeds and Oilseed, 8-10 mars 2004, Toledo, Espagne. Wageningen : Wageningen Academic Publishers, p. 1-4. International Workshop on Antinutritional Factors in Legume Seeds and Oilseeds, 2004-03-08/2004-03-10, Toledo (Espagne).

Experiments were conducted to investigate ileal digestion of legume proteins in piglets weaned onto diets whose protein was provided by casein (control) or an equal protein mixture of casein and raw pea, faba bean, blue lupin, black or white chickpeas, or soya bean meal. heal digesta were analysed by Western blotting with antibodies raised against the main 11S and 7S legume proteins. Only a few immunoreactive legume protein bands were detected by immunoblotting They belonged mainly to the 7S family in the case of pea, faba bean and chickpea. Nearly intact lupin proteins of the 11S family were identified.

Mots-clés : porcin; légumineuse

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