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Fine mapping of a gene for low-tiller number, Ltn, in japonica rice (Oryza sativa L.) variety Aikawa 1

Fujita D., Ebron L.A., Araki E., Kato H., Khush G.S., Sheehy J.E., Lafarge T., Fukuta Y., Kobayashi N.. 2010. Theoretical and Applied Genetics, 120 (6) : p. 1233-1240.

Tillering is one of the most important agronomic traits related to grain production in rice (Oryza sativa L.). A japonica-type variety, Aikawa 1, is known to have low-tiller number. The detailed location of a lowtillering gene, Ltn, which has been localized on chromosome 8 in Aikawa 1, was conWrmed by molecular mapping. Using BC5F2 individuals derived from a cross between IR64 and Aikawa 1, the low-tillering gene was mapped to an interval deWned by SSR markers ssr5816-3 and A4765. This was designated as Ltn because there was no reported gene for tillering in the region of chromosome 8. Through high-resolution linkage analysis, the candidate region of Ltn was located between DNA markers ssr6049-23 and ind6049-1 corresponding to 38.6 kbp on the Nipponbare genome sequence. These DNA markers, which were tightly linked to Ltn, are useful for marker-assisted selection in breeding studies. (Résumé d'auteur)

Mots-clés : pcr; marqueur génétique; identification; gène; génotype; phénotype; amélioration des plantes; tallage; oryza sativa; japon; philippines; france; oryza sativa indica; oryza sativa japonica

Thématique : Génétique et amélioration des plantes; Méthodes de relevé

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