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Isolation and characterization of 15 nuclear microsatellite markers for Baillonella toxisperma Pierre (Sapotaceae), a low-density tree species of Central Africa

Ndiade-Bourobou D., Vaillant A., Favreau B., Gayrin E., Bouvet J.M.. 2009. Molecular Ecology Resources, 9 (4) : p. 1135-1272.

DOI: 10.1111/j.1755-0998.2009.02543.x

Baillonella toxisperma is a scattered endemic species of the Guineo-Congolian forest. We developed 15 nuclear microsatellite markers specifically for B. toxisperma to analyse the genetic diversity of 188 individuals distributed in two natural populations in Gabon. On average, 8.3 alleles per locus were identified; observed heterozygosity ranged from 0.056 to 0.872 and expected heterozygosity from 0.282 to 0.823 among populations. Only three loci showed a significant departure to Hardy-Weinberg equilibrium, but no linkage disequilibrium was observed for any pair of loci.

Mots-clés : sapotaceae; densité de population; marqueur génétique; microsatellite; variation génétique; génétique des populations; gabon; afrique centrale; baillonella toxisperma

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