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Cryopreservation of coconut (Cocos nucifera l.): The influence of embryo maturity upon rate of recovery and fidelity of seedlings

Sisunandar S., Rival A., Samosir Y., Adkins S.. 2010. In Vitro Cellular and Developmental Biology. Animal, 46 : S2-S3. IAPB Congress 2010. 12, 2010-06-06/2010-06-11, Saint-Louis (Etats-Unis).

The genetic diversity of coconut (Cocos nucifera L.) is being lost due to a combination of pest and disease attack and natural disasters. Consequently, there is a need to undertake germplasm conservation before further loss occurs. Since coconut has a large, recalcitrant seed (sensitive to desiccation), it cannot be stored in traditional ways using a seed bank. Cryopreservation is now seen as an important storage approach although available techniques need further improvement. Given the importance of embryo maturity to the success of cryopreservation in other species, the effect of coconut embryo maturity upon cryopreservation success was investigated using two cultivars (Nias Yellow Dwarf and Takome Tall). After cryopreservation, using a new four-step protocol (rapid desiccation, rapid freezing, rapid thawing, and recovery and acclimatization S2 SPEAKER ABSTRACTS for 16 wk in the glasshouse), we found that that embryos isolated from an 11-mo-old fruit gave the highest number of normal seedlings (ca. 28%) when compared to counterparts excised from younger or older fruits. In addition, the results showed that the fruit could be stored for up to 3 wk prior to the isolation of embryos before their performance in cryopreservation was compromised. No morphological and cytological abnormalities were observed in the seedlings recovered from 11-mo-old cryopreserved embryos when compared to their un-cryopreserved counterparts. Genetic and epigenetic testing using microsatellite and global DNA methylation rates could not detect any significant differences between genomic DNA isolated from seedlings recovered from cryopreserved compared to their un-cryopreserved counterparts [S-008]. (Texte intégral)

Mots-clés : cryoconservation; congélation; conservation du matériel génétique; cocos nucifera

Thématique : Génétique et amélioration des plantes; Physiologie végétale : croissance et développement

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