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Brachypodium distachyon as a model system for studying genes involved in cell wall synthesis

Bouchabke-Coussa O., Bouvier d'Ivoire M., Darracq O., Antelme S., Sibout R., Jouanin L., Pollet B., Lapierre C., Dalmais M., Pannetier C., Chupeau Y.. 2010. In Vitro Cellular and Developmental Biology. Animal, 46 : p. S201-S201. IAPB Congress 2010. 12, 2010-06-06/2010-06-11, Saint-Louis (Etats-Unis).

Brachypodium distachyon was proposed as new model plant for grasses because of its small genome and its phylogenetic position between rice and Triticeae crops. Since, a range of genetic and genomic resources has been developed and the whole genome sequence is now available. We use Brachypodium as a model system for studying how to modify grass crops for increased biofuel production made from lignocellulosic cell walls. We are involved in developing many resources for Brachypodium: TILLING collection, natural accessions, different methods of phenotyping, and tools and method for gene transfer via Agrobacterium tumefaciens. Some lignin mutants from the monolignol biosynthesis pathway were already identified by phenotyping and TILLING, and their functional analysis is underway notably through complementation by overexpression of concerned gene in mutant background. (Résumé d'auteur)

Mots-clés : modèle végétal; paroi cellulaire; génome; biosynthèse; biocarburant; brachysporium

Thématique : Génétique et amélioration des plantes; Taxonomie végétale et phyto-géographie; Sources d'énergie renouvelable

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