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Ac-Ds solutions for rice insertion mutagenesis.

Guiderdoni E., Gantet P.. 2012. In : Bigot Yves (ed.). Mobile genetic elements : protocols and genomic applications. New York : Springer [Etats-Unis], p. 177-187. (Methods in Molecular Biology, 859).

DOI: 10.1007/978-1-61779-603-6_10

Rice is the model plant for monocotyledons. Since the completion of the high-quality sequence of its genome, the international community is deploying efforts to identify the function of the 30-40,000 nontransposable element genes of rice. These efforts comprise the creation of large collections of rice mutants accessible to the international scientific community. In addition to loss of function mutants, insertion mutagenesis using Agrobacterium-mediated transformation and engineered mobile elements allows the identification of genes through enhancer or gene trapping or activation tagging. The maize transposable element Ac-Ds is known to be active in rice since the early 1990s and it does not interfere with endogenous rice transposons. This is the guaranty that induced mutation obtained with the mobility of the Ds element will be stable when the source of Ac transposase is removed from the mutated genome. In this paper, we describe single- or double-component T-DNA constructs that have been used to introduce a functional Ac-Ds system in rice and allowed the generation and selection of different type of Ds insertion mutations in the rice genome.

Mots-clés : oryza sativa; génie génétique; mutation provoquée; transposition de gènes; adn; fragmentation de l'adn; marqueur génétique; expression des gènes; transposon

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