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Development of indel markers from Citrus clementina (Rutaceae) BAC-end sequences and interspecific transferability in Citrus

Ollitrault F., Terol J.F., Martin A.A., Pina J.A., Navarro L., Talon M., Ollitrault P.. 2012. American Journal of Botany, 99 (7) : e268-e273.

Premise of the study: Indel markers were developed from BAC-end sequences of Citrus clementina cv. Nules. Transferability and polymorphism were tested in the Citrus genus to estimate the potential of indel markers mined from a single genotype for use in genetic studies. o Methods and Results: Using polyacrylamide gel electrophoresis and DNA silver staining, 89 indel markers were tested for their transferability and polymorphism. Thirty-eight markers were selected. Heterozygosity in C. clementina cv. Nules was confirmed for 33 of these indel pairs. A preliminary diversity study using a capillary electrophoresis fragment analyzer was conducted with 21 indels using 45 accessions representing Citrus genus diversity. Intraspecific and interspecific polymorphisms were observed. o Conclusions: These results indicate the utility of indel markers developed from sequence data of a single genotype of interspecific origin. In Citrus, these markers will be useful for genetic mapping, germplasm characterization, and phylogenetic assignment of DNA fragments. (Résumé d'auteur)

Mots-clés : hybridation intraspécifique; hybridation interspécifique; génotype; Évolution; variation génétique; microsatellite; identification; provenance; germplasm; polymorphisme génétique; rutaceae; phylogénie; carte génétique; marqueur génétique; citrus; chromosome artificiel; ssr; séquencage; citrus clementina

Thématique : Génétique et amélioration des plantes

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