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Story of an array based technology designed to grasp and detect the complex plant pathogen Ralstonia solanacearum

Cellier G., Arribat S., Robène I., Prior P.. 2012. In : QBOL-EPPO Conference on DNA Barcoding and diagnostic methods for plant pests, Haarlem, Netherlands, May 21-25, 2012. s.l. : s.n., p. 40-40. QBOL-EPPO Conference on DNA Barcoding and Diagnosti Methods for Plant Pests, 2012-05-21/2012-05-25, Haarlem (Pays-Bas).

The ancient soil borne plant vascular pathogen Ralstonia solanacearum evolved and adapted to cause severe damage on an unusually wide range of plants. Based on the phylotype classification, it was questioned how genetically and phenotypically diverse strains of Ralstonia solanacearum may be, in an attempt to produce adapted diagnostic tools. A pangenomic microarray was first used to better describe and understand the phylogeny of R. solanacearum, especially three particular ecotypes in the phylotype II: (i) Brown rot strains from sequevars IIB-1 and IIB-2, historically known as race 3 biovar 2 and clonal; (ii) new pathogenic variants from sequevar IIB-4NPB that lack pathogenicity to Cavendish banana but can infect many other plant species; and (iii) Moko disease-causing strains from sequevars IIB-3, IIB-4, and IIA-6, historically known as race 2, that cause wilt on banana, plantain, and Heliconia. Results revealed a phylogeographic structure within Brown rot strains, allowing European outbreak strains of Andean and African origins to be distinguished, and also showed a close but distinct relationship between Moko ecotype IIB-4 and the emerging IIB-4NPB strains. Based on those results, it is proposed to develop new DNA-based diagnostic tools, including array and routine PCR, to ensure better detection of this complex plant pathogen.

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