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Transcriptional response of Pseudomonas aeruginosa to a phosphate-deficient Lolium perenne rhizosphere

Zysko A., Sanguin H., Hayes A., Wardleworth L., Zeef L.A.H., Sim A., Paterson E., Singh B.K., Kertesz M.A.. 2012. Plant and Soil, 359 (1-2) : p. 25-44.

DOI: 10.1007/s11104-011-1060-z

Background and aims Plant-bacterial interactions in the rhizosphere are important in mediating soil nutrient transformations. Plants supply carbon-rich substrates to rhizobacteria as root exudates and bacteria mobilise soil-bound phosphate for plant nutrition. This study aimed to probe the specificity of the plant effect on bacterial gene expression in Pstarved rhizosphere conditions. Methods DNA microarrays were employed to study gene expression in the rhizosphere of Lolium perenne grown under high and low phosphate regimes (330 ?M vs. 3-6 ?M phosphate). Root exudation under these regimes was also quantified. Phosphateregulated gene expression of a panel of 22 genes was compared in rhizosphere, planktonic culture and during biofilm growth on an artificial root. Results Plant growth and root exudation were affected by P-availability. P-limited conditions induced increased expression of bacterial genes of an aromatic degradation pathway (catA), heavy metal sensing (PA2523), and membrane proteins (glpM, crcB), while genes involved in cell motility and amino acid uptake/ metabolism were downregulated. A crcB mutant was impaired in rhizosphere survival under low phosphate conditions, though glpM and catA mutants were not affected. Several of the genes studied were induced by phosphate limitation in all three lifestyles studied. Conclusions Our results show the importance of the plant-microbe interaction in controlling the bacterial transcriptional response in a phosphate-limited rhizosphere.

Mots-clés : lolium perenne; pseudomonas aeruginosa; rhizosphère; interactions biologiques; transcription; phosphate; expression des gènes; génie génétique; carence du sol; puce à adn

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