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Efficient haploid production on 'Wilking' mandarin by induced gynogenesis

Jedidi Néji E., Kamiri M., Poullet T., Ollitrault P., Froelicher Y.. 2015. In : Navarro Luis (ed.), Sabater-Muñoz Beatriz (ed.), Moreno Pedro (ed.), Peña Leandro (ed.). Proceedings of the XIIth International Citrus Congress: International Society of Citriculture. Leuven : ISHS, p. 495-500. (Acta Horticulturae, 1065). International Citrus Congress: Citrus and Health. 12, 2012-11-18/2012-11-23, Valence (Espagne).

Seedlessness is a major criterion for the citrus fresh fruit market. Therefore, triploid breeding appears very promising for the selection of new citrus cultivars. CIRAD has developed a method to produce triploid hybrids by somatic hybridization between diploid and haploid lines, and demonstrates the potential for haploid citrus production by induced gynogenesis in clementine. In this work we analysed the efficiency of induced gynogenesis by irradiated pollen in 'Wilking' mandarin (Citrus reticulata). Five doses of gamma irradiation of 'Meyer' lemon pollen were tested (0, 150, 200, 250, 300 Grays). Embryos were extracted from small seeds, obtained in immature fruits, and cultivated in vitro. The ploidy level of plantlets was determined by flow cytometry analysis. Irradiated pollen appeared to be efficient for gynogenesis induction in 'Wilking'. A relatively high number of haploids (7 plantlets) were obtained, essentially after hybridisation with 'Meyer' lemon pollen irradiated at 250 Grays. The ploidy level of all haploid plantlets was confirmed by chromosome counts, and molecular markers demonstrated their gynogenetic origin. These haploid lines should be useful for triploid breeding by somatic hybridisation, and also constitute interesting resources for genomic studies.

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