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Root protein interatomic network obtained from citrus seedlings subjected to water deficit : S08O03

Magalhaes de Oliveira T., Silva F., Morillon R., Coelho Filho M.A., Neves D.M., Costa M.G.C., Pirovani C.P., Bonatto D.. 2012. In : Navarro Luis (ed.). XII International Citrus Congress : Book of abstract. Riverside : ISC, p. 117-118. International Citrus congress "Citrus and health. 12, 2012-11-18/2012-11-23, Valence (Espagne).

Citrus Brazilian industry may be strongly affected by drought. Characterize rootstock behavior under different environmental conditions is crucial, since rootstocks may confer better adaption to environmental stresses. Plant responses to drought require the production of important functional and regulatory proteins. In the present study, we identified proteins related to water deficit response in tolerant ('Rangpur' lime, Citrus limonia) and susceptible ('Sunki Maravilha' mandarin, Citrus sunki) genotypes. From 105 differentially expressed proteins, twenty-nine proteins from roots of both genotypes were identified and sequenced by mass spectrometry. Protein interaction analysis of orthologous proteins from A. thaliana protein expressed in the two citrus varieties was obtained by ontological and cluster analysis.Among the 16 amino acid sequences found in 'Rangpur' lime and 'Sunki Maravilha' mandarin, 13 proteins were found in A. thaliana via BLAST, of which, 12 showed protein-protein interaction. It is possible to group these 12 proteins into a single network interatomic, comprising 3302 proteins. Among the different biological processes found in the protein networks, regulation of response to water deficit and osmotic stress, signaling pathway of abscisic acid were highlighted. A point to take into account is the importance role of the DREB2, a protein hub/bottleneck which participate in the stimulus and the response to water stress. (Texte intégral)

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