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Induced systemic resistance (ISR) to control Rotylenchulus reniformis in pineapple

Soler A., Corbion C., Marie Alphonsine P.A., Quénéhervé P.. 2012. Nematropica, 42 (2) : p. 372-372. ONTA Annual Meeting. 44, 2012-09-02/2012-09-07, Cancun (Mexique).

Pineapple infestations by the reniform nematode Rotylenchulus reniformis cannot be managed anymore in the French West lndies. The new European regulations forbid the use of nematicides therefore we looke.d for a possible !SR contribution to nematode biocontrol in pineapple. In gre.enhouse trials, methyljasmonate (10-4M) or water (control) treatrnents were applied over the root system of pineapple plante.d either in IL single pot or in twin-pots (split-root system). In the single pot trial, a monospecific inoculation of 5,000 juveniles of R. reniformis has been made and population increase and the fecundity were monitore.d after 2 months. In the second experirnent 25,000 juveniles were inoculate.d in one side of the twin-pots. On the other side, enzymatic indicators of stress (LOX, SOD, PAL, and CHI) were measure.d at 0, 1, 4, 10, and 14 hours after tre.atments on non-inoculated plants. The !SR ' priming' effect was me.asured with the same indicators 10 d.ays after the inoculation of treated plants. In the first trial, the fecundity was strongly reduced by the methyljasmonate (50 to 70%,p=0.003). In the split-root trial, the .data support the evidence of an !SR set up by the plants as we observed a systemic and transient stress response of the plant to the methyljasmonate. We also observed a 'priming· effect on the plant defense against the nematodes characterized by a significant increase of the enzymatic indicators. These results support the idea of a possible !SR contribution to a R. reniformis biocontrol. (Texte intégral)

Mots-clés : insecticide; lutte antiravageur; méthode de lutte; activité enzymatique; mécanisme de défense; expérimentation en laboratoire; ananas comosus; rotylenchulus reniformis; antilles françaises; martinique

Thématique : Ravageurs des plantes

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