Culicoides are small biting midges involved world wide in bluetongue and African horse sickness transmission. Feeding behaviour as well as spatiotemporal dynamics of the trophic behaviour of Culicoides is unknown at the specifie level for palaearctic species. Multiplex allele-specific peR assays were used to identify the blood meal source of five main palaearctic species. Species-specific primers of potential hosts (cow, goat, sheep, human, cat, dog, horse) were derived from vertebrate mitochondrial cytochrome b. Two different multiplex assays allowed the identification of blood meals from livestock or domestic fauna. It also enabled us to identify whether multiple blood meals had been taken (gonotrophic concordance). Moreover, this me\hod allows identifying specimens at the specifie level. The first results highlight the interest of this valu able tool combined with species identification assays, and suggest that Culicoides species may have an opportunistic behaviour regarding the host distribution and density. Future studies will focus on the temporal dynamics of trophic behaviour.