The production of natural fibers is not sufficient to accommodate the textile needs of the growing world population. Therefore, textile research is exploring alternative natural resources to produce fibers. Though typically known for its nutritional use, the sugarcane can also be used for textile production because of its high fiber content. The aim of our study was to extract fibers from sugarcane and to analyze their mechanical behavior. Cane particles were treated with an alkaline solution in order to get cellulosic fibers. Physical and mechanical characterizations were carried out on these fibers: linear density, fineness, tensile properties and bending rigidity. Their micro-structure was analyzed to better understand their behavior. The results showed a strong influence of extraction parameters on the characteristics of fibers. Depending on these parameters, fibers fineness ranged from 8 to 80 tex, length ranged from 19 to 62 mm, and tenacity ranged from 7 to 25 cN/tex.