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RNAseq analysis of different Hevea genus inoculated by Microcyclus ulei : S01P09

Rippel Salgado L., Koop D.M., Magalhaes M., De Vasconcelos A.T.R., Silva W.A., Garcia D.. 2013. In : Brasileiro Ana Christina Miranda (ed.), Fortes Ferreira Claudia (ed.), Fernandez Diana (ed.), Micheli Fabienne (ed.), Coelho Filho M.A. (ed.), Marraccini Pierre (ed.). Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century : Book of abstracts of the CIBA 2013. Brasilia : EMBRAPA, p. 41-41. Workshop on Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century, 2013-11-06/2013-11-08, Ilhéus-Bahia (Brésil).

The rubber tree, Hevea is native from the Brazilian Amazon region and it is responsible for almost all natural rubber produced in the world, a strategic raw material for a variety of products. One of major challenges for rubber tree cultivation is the adaptation to biotic stress caused by several leaf pathogens. Microcyclus ulei, causal agent of the South American leaf blight (SALB) is responsible of massive losses of leaves by infection, leading to a low latex production and sometimes leading to plant death. The main proposed strategies to avoid the M. ulei damaging on plantations are to cultivate productive and SALB resistant genotypes. In Hevea, quantitative and qualitative resistance to M. ulei can be found in cultivars and but also between genus. By transcriptomic comparison of RNAseq of three genus of Hevea (H. brasiliensis cv. MDF180 and cv. PB314, H. pauciflora cv. PA31 and H. benthamiana cv. F4542), our purpose was to identify genes differentially expressed and characteristic of each genus. Hevea leaf lesions and non-inoculated controls were collected 24, 48, 96 and168 hpi. For each cultivar inoculated or non-inoculated, total RNA of time samples were pooled and enriched in mRNA. The eight cDNA libraries were sequenced with 454 XL+ sequencing kit and produced a total 4,225,291 reads de novo assembled on 28,349 contigs. A tBLASTx against a NCBI plant RefSeq was performed and 15,442 sequences were successfully annotated. A differential expression analysis was conducted to each library with the NOIseq 2.0 software (Bioconductor) and for a cutoff of q score of 0.9, we identified in F4542 cultivar (quantitative resistance) 89 up-regulated and 32 down-regulated genes, in MDF180 cultivar (quantitative resistance), 117 up- and 71 down-regulated genes, in PA31cultivar (quantitative resistance), 72 up- and 12 down-regulated genes and in PB314 cultivar (susceptible) 455 up-regulated and 169 down-regulated genes. This preliminary analysis provides a first look at the host and parasite transcriptome of Hevea spp. Further analyses are underway to distinguish the host genes from those of the parasite and identify with more accuracy the genes differentally expressed in each Hevea genus during the infection. Work supported by CAPES/CNPq. (Texte intégral)

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