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Development of microsatellite-enriched libraries

Vignes H., Rivallan R.. 2014. In : Besse Pascale (ed.). Molecular plant taxonomy : methods and protocols. Clifton : Humana Press, p. 177-189. (Methods in Molecular Biology, 1115).

Among the molecular markers used for plant genetic studies, microsatellite markers are easy to implement and can provide suitable codominant markers for molecular taxonomy. Here we describe a method to obtain enriched libraries in microsatellite loci from genomic DNA, using capture method with synthetic oligonucleotide probes. (Résumé d'auteur)

Thématique : Génétique et amélioration des plantes; Méthodes de relevé

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