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DNA and RNA polymerase activity in a Moniliophthora perniciosa mitochondrial plasmid and self-defense against oxidative stress

Andrade B.S., Villela Dias C., Gomes D.S., Micheli F., Goes-Neto A.. 2013. Genetics and Molecular Research, 12 (2) : p. 1944-1950.

DOI: 10.4238/2013.June.13.3

Moniliophthora perniciosa (Stahel) Aime and Phillips-Mora is a hemibiotrophic basidiomycete (Agaricales, Tricholomataceae) that causes witches' broom disease in cocoa (Theobroma cacao L.). This pathogen carries a stable integrated invertron-type linear plasmid in its mitochondrial genome that encodes viral-like DNA and RNA polymerases related to fungal senescence and longevity. After culturing the fungus and obtaining its various stages of development in triplicate, we carried out total RNA extraction and subsequent complementary DNA synthesis. To analyze DNA and RNA polymerase expression levels, we performed real-time reverse transcriptase polymerase chain reaction for various fungal phases of development. Our results showed that DNA and RNA polymerase gene expression in the primordium phase of M. perniciosa is related to a potential defense mechanism against T. cacao oxidative attack.

Mots-clés : theobroma cacao; moniliophthora; mécanisme de défense; oxydation; stress; plasmide; mitochondrie; transcription inverse; activité enzymatique; expression des gènes; brésil; moniliophthora perniciosa

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